Genome wide gene expression profiling demonstrated that mouse ESC and iPSC differentiated osteoblast progenitors resemble osteoblasts

We differentiated mouse Col2.3 GFP ESCs and iPSCs to osteoblast progenitors and confirmed the osteoblast identity of ESC- and iPSC-derived osteoblasts using RNA-sequencing and hierarchical clustering, and demonstrate that Col2.3GFP+ sorted cells are more similar to freshly isolated Col2.3GFP+ osteoblasts from mouse bone than to unsorted cells based on whole genome expression profiling. Overall design: ESC- and iPSC-derived osteoblasts, and Col2.3GFP+ sorted cells and freshly isolated Col2.3GFP+ osteoblasts