A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation

We hypothesized a convergence of transcriptome profiles in hypocotyls of low blue light (LB) or low red/far red light (LRFR) treated seedlings as both conditions trigger hypocotyl cell elongation. To test this hypothesis, we did a transcriptome analysis in of 5d-old long-day (LD)-grown Col-0 (wild type, WT), pif457, and yuc2589 seedlings treated for 3 hours with LB, LRFR or remaining in the same light condition (WL). We used dissected cotyledons and hypocotyls to characterize the LB and LRFR response in both organs and determine their dependency on PIFs and YUC-mediated auxin biosynthesis. Overall design: Total mRNA profiles of 5 day old Arabidopsis cotyledons and hypocotyls from three genotypes: Col-0 (WT), pif457, yuc2589, and smt2-1, which were subjected to three light conditions: white light (WL), low blue (LB) and low red/far red (LRFR).