JAL-TA9 and GSGFR cleaves amyloid beta fragment peptides

Time-dependent analysis of the reaction mixtures of JAL-TA9 and Aβ11-29. 0.05 mM Aβ11-29 was incubated either (a) without or (b) with 0.2 mM JAL-TA9. 0.05 mM Aβ11-29 was incubated either (c) without or (d) with 0.2 mM GSGFR.The reaction mixtures were analyzed by HPLC. Peaks observed after incubation were fractionated and applied by ESI-MS analysis. Cleavage sites were summarized (e) and (f).

搜集汇总

背景与挑战

背景概述

该数据集记录了JAL-TA9和GSGFR两种肽酶对淀粉样β片段肽Aβ11-29的切割作用。通过时间依赖性HPLC分析反应混合物，并结合ESI-MS鉴定裂解产物，总结了两种酶在Aβ11-29上的切割位点。