SARS-CoV-2 infection elucidates unique features of pregnancy-specific immunity [GEX]

Pregnancy is a risk factor for increased severity of SARS-CoV-2 and other respiratory infections. The mechanisms underlying this risk have not been well-established, partly due to limited understanding of how pregnancy shapes and modulates immune responses. To gain insight into the roles of pregnancy in shaping the immune responses we collected peripheral blood mononuclear cells (PBMC) from 17 non-pregnant and 9 pregnant donors with various COVID severities and compared their immunological profiles. Hashed PBMC single cell libraries were generated with the Chromium Single Cell 5’ kit (10X Genomics) together with the 5’ Feature Barcode library kit. TCR and BCR-enriched cDNA libraries were generated with the Chromium Single Cell V(D)J Enrichment kit (10x Genomics). Library quality was assessed with an Agilent 2100 Bioanalyzer. >>>Submitter states: Due to patient privacy concerns, raw data will be submitted to dbGaP.<<<