Atopyrelated immune profiles are subject to genetic influence as evaluated using school-aged twin pairs

Both genetic and environmental risk factors have been described for atopic disease. However, the interaction of these with individual immune profiles remains unclear. Using flow cytometry, immune profiles of 93 school-aged twin pairs as well as in vitro responses were determined to evaluate immunological responses associated with atopy. Findings suggest a genetic impact on immune cell abundance, particularly for B cells. Whereas cytokine responses from in vitro stimulations appeared mainly shaped by environmental exposures. Relating to atopic traits, we observed increased abundance of both B cells and basophils in atopic individuals as well as increased expression of the IgE receptor across several subsets, including basophils and dendritic cells. Genetic background appeared central to regulating IgE receptor expression on basophils whereas expression on dendritic cells instead appeared sensitive to environmental exposures. Identifying environmentally regulated immune traits may facilit..., , # Atopy-related immune profiles are subject to genetic influence as evaluated using school-aged twin pairs

Dataset DOI: [10.5061/dryad.0gb5mkmch](10.5061/dryad.0gb5mkmch)

## Description of the data and file structure

**Study cohorts**

A subset of school-age individuals (age range 7-13 years) from the Western Australian Twin Study of Child Health (WATCH). A total of 93 twin pairs (186 individuals) were included in the present study.

**Clinical parameters**

Atopy was defined as either (i) a positive skin prick test (SPT, wheal size ≥3mm) for any of the most common aeroallergens including house dust mite (HDM), cat dander, dog dander, fungi (*Alternaria alternata*, *Aspergillus spp.*), cockroach, perennial rye grass, mixed grass, and mixed mould or (ii) a total IgE level above 300 IU/mL as measured by a clinical pathology laboratory in Australia.

**Flow cytometry analysis**

Approximately 1x10^6 thawed PBMCs were stained. Briefly, thawed PBMC were washed using PBS supplemented with ..., All participants have consented to that their research data will be published and the data has been de-identified prior to submission into the public domain.