RNA sequencing in Chrysanthemum morifolium

Express sequence tag (EST) sequencing is important for functional analysis in especially in horticultural plants whose genome information is lacking. Therefore, we think that our aim in this project is obtaining sufficient information of ESTs for functional analysis on gene(s) of interest in chrysanthemum. We performed largescale transcriptome analysis using a next-generation sequencer in chrysanthemum using 454 sequencing system. We constructed normalized cDNA libraries, consisting of full length, 3''-UTR, and 5''-UTR cDNAs derived from various tissues of Chrysanthemum morifolium cultivar ''Sei-Marin'' for the 454 sequencing. In these chrysanthemum ESTs, a series of metabolic genes of petal colors and fragrances, and genes of TF families which have been reported in Arabidopsis, were mostly contained.