Transcriptome analysis of NK92 treated with deferoxamine or PPMX-T003

Although several types of iron deprivators are available in laboratory, the differences of them in transcriptome alterations remains unclear. In this study, we performed RNA-seq analysis of NK92 cells treated with deferoxamine (DFO; an iron chelator) and PPMX-T003 (an anti-transferrin receptor 1 inhibitory antibody) to investigate the differences in transcriptomic alterations between them. NK92 cells were treated with 500 µM of DFO or 10 µg/mL of PPMX-T003 for 24 hours, followed by RNA extraction using Qiazol. cDNA library was constructed using TruSeq Standard mRNA Library Prep. Sequencing was performed using NovaSeq-6000 with 101 bp paired end mode. Reads were mapped into hg38 using HISAT2 version 2.2.1 after trimmed with fastp version 0.21.0, followed by conting them using StringTie version 2.1.6 with RefSeq gene annotation file of hg38.