Screening for potential target genes of microRNA-548ac using HTA 2.0 microarrays. Screening for potential target genes of microRNA-548ac using HTA 2.0 microarrays

We screened for target genes of hsa-miR-548ac by transcriptome profiling. The miRNA was overexpressed in HeLa cells by transient transfection of a precursor miRNA expression plasmid. High-density Affymetrix Human Transcriptome Arrays (HTA) 2.0 were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow. Overall design: HeLa cells were transiently transfected with a non-viral precursor miRNA expression plasmid (HmiR1085-MR04, GeneCopoeia). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. RNA samples were obtained 24 h and 48 h after transfection of the mir-548ac plasmid (n=3 replicates) or the control plasmid (n=2 replicates). For all 10 RNA samples, transcript levels were measured with Affymetrix HTA 2.0 microarrays.