Features of different methyltyping techniques.

For definition of spanning and determination of number of sites, see Methods. Constraints on analysis: for MethylSeq, see this paper. For RRBS, bisulphite conversion lowers sequence complexity, complicating alignments. Affinity based methods are complicated by effects of methylation density on binding, and by noise created by non-specific binding. Array methods are complicated by noise in the hybridization step. This table makes use of information from Laird 2010 [26].