The sequencing results of PCR products
收藏科学数据银行2022-01-11 更新2026-04-23 收录
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资源简介：
We chose 17 representative species for confirming the IR region expansion/contraction. The four junctions between LSC/IRs and SSC/IRs of the 17 species were confirmed with PCR-based product sequencing. Target DNA regions were amplified in 25 μl reactions containing 10 ng (1 μl) template DNA, dNTP mixture 2 μl, 10 × LA PCR Buffer 2.5 μl, 0.5 μl of each primer, and 18.5 μl ddH2O. PCR products were bidirectionally sequenced by GENEWIZ, Inc.
提供机构：
South China Institute of Botany
创建时间：
2022-01-10
