Gene expression profile at single cell level of cells from cholangiocarcinoma(CCA) and adjacent tissues.

Multiple tumor-associated cells in the complex CCA microenvironment.We used single cell RNA sequencing (scRNA-seq) to analyze the gene expression diversity of cells in the CCA and adjacent tissues. Overall design: After dissociating tumor and adjacent tissues, lysing red blood cells and removing dead cells, candidate cells were scaptured to analyze used scRNA-seq.