Single-cell RNA sequencing of proliferative stem cell population from juvenile Schistosoma mansoni worms

We applied Smart-Seq2 protocol to sequence 370 single stem cells isolated from 2.5 weeks old Schistosoma mansoni worms, which infect millions of people worldwide. We discovered new stem cell sub-populations using a novel single-cell RNA sequencing analysis method (Self-assembling manifolds, SAM). We found differential expressions in a few transcription factors and marker genes that distinguish these sub-populations. We hope this will be a useful resource for the community interested in the developmental biology of Schistosoma at large.