A phylogenetic analysis of Marcetia (Melastomataceae, Marcetieae) and three new sprawling species from Bahia, Brazil

This dataset contains:

-A fasta file containing DNA sequences for 107 species of Marcetieae (Melastomataceae). This alignment was imported into RAxML 8.2.12 (Stamatakis 2014) and used in phylogenetic analyses.

-Binary images of leaves and anther connectives of Marcetia alba, M. barbadensis, M. minima, and M. serratifolia. These images were used to generate outlines for the shape analyses in Momocs.

Methods
DNA alignment
-Total genomic DNA was extracted from silica-dried leaves or herbarium specimens. We amplified and sequenced the ribosomal markers ETS and ITS and the chloroplast markers accD-psaI, atpF-atpH, trnS-trnG, psbK-psbL, ndhF and rbcL. We sampled a total of 107 terminals comprising 107 species. The sampling included 35 species of Marcetia (ingroup), plus 72 species from the other 18 genera of Marcetieae (outgroups).

The ends of all matrices were trimmed in Mega X (Kumar et al. 2018). Each marker was aligned separately using the package DECIPHER (Wright 2016) implemented in the R

The concatenated sequences were analyzed using Maximum likelihood (ML) in RAxML 8.2.12 (Stamatakis 2014) with the GTR+Γ model, partitioned by region, and employing the ‘-f a’ setting in the configuration file.

Binary images
-For obtaining outlines, high-resolution photographs of mature leaves (n=10 for each species) and anther connectives in lateral view (n=5) were taken using a stereoscope with an attached camera. Based on these photographs, we used GIMP 2.10.32 (http://www.gimp.org) to generate binary images with the leaves and connectives as black shapes on a white background and export them as jpg files.