The rye B chromosome drive:WGS

The B chromosome is a good example to learn the non-Mendelian inheritance. It has been nearly a century since scientists found that the rye B chromosome undergoes targeted nondisjunction during the first pollen mitosis (PMI) to increase its number into the next generation. However, the genetic elements that control this process are poorly understood. To identify the drive-controlling trans-acting factor(s) of the rye B chromosome, first, we generated a preliminary assembly of the rye B chromosome using PacBio® HiFi sequencing. To better understand how the genetic elements control the drive of the rye B chromosome, we decided to assemble the sequences of the rye B chromosome. Combining HiFi and ONT reads, hifiasm (Ultra-long ONT integration mode) (Cheng et al., 2023) was used to assemble the whole genome of wheat with rye B chromosomes.