The Transcriptome of MHV or PVM Infected RAW264.7 Cell

In the current in vitro research, murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus, a coronavirus existed in mouse. The transcriptome analysis showed the differential expressed genes(DEG) related to important cellular physiological activity. In addition, DEGs related to metabolism and histone modification were detected, which might correlate with the trained immunity of macrophages. The current in vitro viral infection study showed the key innated immunity character of macrophages, which suggest the replacement value of viral infection cells model, to reduce the animal usage in preclinical researches. Cells from ATCC were cultured in DMEM medium with 10% fetal bovine serum (FBS) at 37 °C with 5% CO2. Before viral infection，the medium was changed to DMEM with 1%FBS. 800 TCID50 were added per well. At 3, 6, 12, 24, 48hour post infection(hpi.), the culture medium was discarded, and the attached cells were washed with PBS and harvested in Trizol reagent.Transcriptome sequencing was carried out byNovo Zhiyuan Co., Ltd. (Beijing, China).