P. aeruginosa PAO wildtype Cy3 PA3898 mutant Cy5. P. aeruginosa PAO wildtype Cy3 PA3898 mutant Cy5

We present here a transcriptional regulator, PA3898, which controls biofilm formation and multidrug efflux-pumps in P. aeruginosa. A mutant of this regulator significantly reduced the ability of P. aeruginosa to produce biofilm in vitro, and affected its in vivo fitness and pathogenesis in Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA3898 modulates essential virulence genes/pathways, including multidrug efflux-pumps and phenazine biosynthesis. Overall design: PAO1 wildtype versus PA3898 mutant both grown in LB media. cDNA samples were hybridized onto P. aeruginosa 70mer duplicate array (GEO accession number GPL10633).