Normalized peak area data of the compounds in media and metabolites of Salmonella enterica serovar Typhimurium 14028s and aceB mutant

The abundance of major carbon metabolism intermediates in media of MgCl2, minimal medium (MM), diluvial sand (DS) soil suspension, tomato leaf-based medium (TM), and lettuce leaf based medium (LM) was measured using GC/MS. Correspondingly, the abundances in the cells of _Salmonella enterica_ serovar Typhimurium 14028s and _aceB_ mutant culrtured in such media were determined. _Salmonella_ culture in stationary phase was centrifuged, washed, and diluted in 10 mM MgCl2 (10^9 CFU/mL). Three milliliters of the dilution were added into a cellulose ester dialysis membrane (MWCO 100 000, φ=16 mm, Spectra/Por Biotech, USA) bag, which was then sealed and immersed in 30 mL of the respective medium in a 50 mL centrifuge tube. The system was shaken at 28°C for 24 h. In the metabolism experiment, part of the samples was separated for bacterial enumeration, while the rest was pelleted at 4°C in a precooled centrifuge tube. After being rinsed on ice with cool 10 mM MgCl2, the pellets were low-temperature centrifuged again and frozen in liquid nitrogen. Metabolites were extracted from wet biomass by methanol-water-chloroform extraction using 100 µL methanol/13C-ribitol solution to resuspend the cells. Thereafter, sequential addition of 100 µL H2O and 150 µL chloroform was performed with vortexing of 5 min in between. After centrifugation, 150 µL of the polar phase was dried in a vacuum concentrator. For extracellular metabolites, 10 µL of the cell free culture supernatant were mixed with 10 µL methanol/13C-ribitol solution and dried in a vacuum concentrator. Derivatization and GC-MS measurement (splitless and split 1:10) of metabolites was performed as described in Will et al. (2019). Processing of raw data was performed as described in Neumann-Schaal et al. (2015).