Aggregation tendency indicated by cell counting analysis.
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COS cells were transfected with various ataxinCT constructs fused with polyQ repeats or repeats containing His interruptions. 48 h after transfection, cells were stained and analysed by fluorescent microscopy and the percentage of cells with aggregates was determined. Approximately three hundred cells were counted in each experiment. Data represent means ± SD of at least three experiments. Asterisks indicate sequence patterns corresponding to those identified in patient samples.
创建时间:
2015-12-02



