Changes in carbohydrate metabolism in alveolar epithelial type II cells in Ub-Cre and Hif1a loxp/loxp Ub cre mice in respnse to injurious mechanical ventilation (IMV). Changes in carbohydrate metabolism in alveolar epithelial type II cells in Ub-Cre and Hif1a loxp/loxp Ub cre mice in respnse to injurious mechanical ventilation (IMV)

IMV induces transcription of key glycolytic enzymes in alveolar epithelial type II mice IMV induced transcription of key glycolytic enzymes is HIF1A dependent Overall design: Mice were subjected to IMV (peak inspiratory pressure 45, PEEP 3, RR 80, FiO2 100%) or control ventilation (peak inspiratory pressure 12, PEEP 3, RR 80, FiO2 100%) , duration of mechanical ventilation 4 hours. After that alveolar type II epithelial cells were isolated form lungs