Lining macrophages initiate articular inflammation

This dataset contains the transcriptional analysis of lining macrophages and sub-lining macrophages from vehicle or antigen-induced arthritis(AIA) mouse model. mice were immunized by two subcutaneous injection of 20 µg mBSA (40 mg/ml) emulsified with CFA (3.3 mg/ml) and PBS to a final 100 µl volume per mouse. Two hundred lining and sublining macrophages from 3 WT and 3 IRF5KO mice, isolated from PBS-Mbsa injected knees. Sorting of synovial macrophages was carried out from the knee cell suspensions. Lining macrophages were defined as CD45+, Linage- (NK1.1, CD3, CD19), CD11b+, F4/80+ and VSIG4+ cells, whereas sublining macrophages were CD45+, Lin-, CD11b+, F4/80+ and VSIG4-.