RNA Seq analysis following 1X or 3X Male Courtship Suppression training in Drosophila

To obtain a comprehensive view of genes contributing to long-term memory we performed mRNA sequencing from single Drosophila heads following behavioral training that produces long-lasting memory. Virgin males exposed to unreceptive females for one or three two-hour sessions separated by 30 minutes were collected one hour after training for comparison to mock trained flies, with six replicates for each condition, for RNA-Seq analysis of the fly heads with an Illumina HiSeq 2500.