Single-cell RNA-seq of axolotl regenerating and control thymus

For 5 dpt, 10 dpt, 21 dpt and 35 dpt samples for scRNA-seq the whole region of thymectomy was collected to include the thymic nodules and surrounding tissues using dissection forceps and scissors. For the control and regenerated thymic nodules at 60 dpt only the thymic nodules themselves were collected excluding any additional surrounding tissue. Six thymic nodules or six thymic area samples were pooled per condition