MicroRNA analysis of Lu134A cells following ASCL1 knockdown

Molecular subtypes of SCLC have been defined by the expression status of ASCL1, NEUROD1, YAP1, and POU2F3 transcription regulators. ASCL1 knockdown resulted in decreased and increased expression of miR-375 and miR-455-3p, respectively. Analyses of publicly available transcriptome datasets suggested that miR-375 induced by ASCL1 is involved in YAP1 suppression whereas miR-455-3p is higher in non-neuroendocrine SCLC cells lacking ASCL1 expression. Lu134A cells were trasfected with siRNAs for ASCL1, and microRNA array was performed using Affymetrix GeneChip® miRNA 4.0 Array.