single-nucleus transcriptomic analysis of cortical tissue from control and Dot1l HET 10 week old mice.

We aimed to understand the transcriptional impact of monoallelic loss of DOT1L in cortical nuclei from 10 week old mice. Overall design: Control and DOT1L HET cortical nuclei were isolated from 10-week-old mice. DOT1L HET mice were heterozygous for floxed DOT1L and both Controls and DOT1L HET were heterozygous for CMV-cre (Strain #:006054). Cortical nuclei were isolated and prepared using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression reagents. (n = 6 biological replicates combined per sample/genotype, 3 male mice and 3 female mice)