Cochliomyia hominivorax Transcriptome or Gene expression

The RNA-seq method can be used for non-model species which do not have an annotated genome or transcriptome. However, the standard RNA-seq strategy has one limitation: longer transcripts are broken into more fragments than the shorter ones. Longer transcript are therefore represented by more reads. This results in an inaccurate estimation of the expression levels of the longer transcripts. The alternative 3''RNA-seq method which yields only one sequence per transcript (the 3''region) was conceived to bypass this limitation.