Allen Institute Openscope - Responses to inconsistent stimuli in somata and distal apical dendrites in primary visual cortex

This dataset was collected for the Credit Assignment project, as part of the Allen Institute for Brain Science's OpenScope project. The most up-to-date links to the publications associated with this dataset (a dataset descriptor paper and an analysis paper) can be found by going to the metadata Github repository, linked below.

Briefly, mice were habituated to head-fixation on a running disc over a period of 11 days. Over the last 6 days, mice were presented with repeating stimulus sequences, with consistent features. These sessions are not included in the dataset, as imaging was not performed. Following habituation, sequences with inconsistent features were introduced over three sessions, while two-photon calcium imaging was performed in pyramidal neurons of primary visual cortex (VisP). Specifically, recordings for each mouse were made in one of four planes: L2/3 somata, L5 somata, L2/3 distal apical dendrites or L5 distal apical dendrites. As mice gained experience with the inconsistent sequences, responses to these events were found to evolve in opposite ways in the dendritic and somatic compartments.

The dataset includes 50 sessions total, recorded in 13 subjects, with at least 3 sessions per subject. Each session file includes: **(1)** ROI dF/F traces, **(2)** ROI masks, **(3)** ROI tracking information (where applicable), **(4)** running velocity traces, **(5)** pupil diameter traces, **(6)** pupil centroid position traces in x and y, **(7)** stimulus parameters. Note that gaze was not computed for this dataset. What is provided is the position of the pupil centroid in the pupil recording videos. In addition, a second, slightly larger version of each file was created, also including: **(8)** stimulus frame images (identifiable by `+image` in the file name). Lastly, a third, much larger version of each file was created, also including: **(9)** the motion corrected imaging stack (identifiable by `_obj-raw` in the file name).

Certain sessions in this dataset were excluded from our analyses for quality control reasons. They are provided here, as the reasons we excluded them from our analyses may not pose a problem for all use cases. The metadata Github repository, linked below, provides detailed information about each session.

Release notes for each version of the dataset are provided in the CHANGES file.