Extracellular proteolytic activity in different clinical isolates of Fonsecaea pedrosoi.
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aThe proteolytic activity was quantified in the absence (control) or in the presence of pepstatin A in the standard assay described in Experimental Procedures section using BSA as soluble proteinaceous substrate, 10 mM citrate buffer, pH 4.0, and 20 µl of each concentrated supernatant (equivalent to 5 µg of protein). The proteolytic activity was expressed as arbitrary unit (AU).
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2015-12-02
