Dynamics of calcineurin-regulated gene expression in the absence of Hog1 in S. cerevisiae

Gene expression was quantified in crz1 hog1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. We show that calcineurin-dependent downregulation of G1/S genes is partly dependent on the osmostress-activated kinase Hog1. For each strain/condition four samples were collected at intervals through 60 minutes following the addition of CaCl2. Duplicate time course experiments were performed for each strain/condition.