Fast 3D Clear: A Fast, Aqueous-Based, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body

To date a variety of Tissue Clearing methods have emerged as powerful tools to study intact whole organ anatomy and neuronal brain circuits. However, most of the current clearing methods are time consuming and require the use of several organic solvents rendering the tissues incapable of further processing other than light sheet microscopy. Here we developed a new tissue clearing method named Fast Clear. This water-based clearing method requires a small number of organic agents, provides transparent adult mouse brains in short time (less than 3 days) preserving any transgenic or viral/dye fluorophore and is completely reversible maintaining the integrity of the tissue making compatible with any other clearing method and immunohistochemistry

迄今为止，多种组织透明化方法已崭露头角，成为研究完整器官解剖结构和神经元脑回路的有力工具。然而，当前大多数透明化方法耗时较长，且需要使用多种有机溶剂，导致组织无法进行除光片显微镜以外的进一步处理。鉴于此，本研究开发了一种名为Fast Clear的新型组织透明化方法。该基于水溶液的透明化方法仅需少量有机试剂，能够在短时间内（不超过3天）提供透明成年小鼠脑组织，并保持任何转基因或病毒/染料荧光素的存在，且完全可逆，维持组织结构的完整性，使其与其他透明化方法和免疫组化技术兼容。