Proteomic Analysis of LncPTEN1 RNA Pulldown in Lung Cancer Cells Using LC-MS/MS

This study aims to identify the molecular function of LncPTEN1 in lung cancer and explore its associated proteins and pathways. Using RNA pulldown coupled with mass spectrometry, we investigated the protein interactions of LncPTEN1 in A549 lung cancer cells. The experiment included three biological replicates with samples 1, 2, 3 as control group and samples 4, 5, 6 as LncPTEN1 overexpression group. The study revealed potential binding partners including Vimentin and Trim16, providing insights into the regulatory mechanisms of LncPTEN1 in lung cancer metastasis.