RNA-seq of Clostridioides difficile ?rpoN mutant against wild-type control

The experiment intends to reveal the difference in gene expression profiles between the wild-type strain and the ?rpoN mutant of Clostridioides difficile. We first constructed the ?rpoN mutant, and the phenotypic changes of the ?rpoN mutant against the wild-type strain were studied. To further elucidate the mechanism of phenotypic changes of the ?rpoN mutant, RNA-sequencing experiments were carried out to reveal the underlying mechanism of phenotypic changes.