Multiplexed fluorescence imaging based on cycles, raw and processed data.

This dataset was created from a larger acquisition in order to provide an example of reasonnable size, as a companion data set to the F1000Research paper preprint DOIXXX. The original raw data including metadata files are included in <strong>Microscope_Output.zip.</strong> <strong>Experiment.json</strong> and<strong> channelnames.txt </strong>are the ones generated by the acquisition software. They are the only files needed when starting from one of the processed data set below. The deconvolution obtained with the commercial software Microvolution is also provided in <strong>bu_deconvolution.zip.</strong> To start from Step 1(Extended Depth of Field) instead of Step 0 (deconvolution), unzip this file in your output directory and rename the folder bu_deconvolution to out. The extended field of view 2D images created from step 0 to step 2, provided for convenince in <strong>edfonly.zip</strong> The final files generated by trhe Multiplex processor, including the segmentation mask , are provided in<strong> finaloutput.zip</strong>. These files can be used in a specific analysis software.