DATASET - Mass Spectrometry - Snake venom proteomics of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Türkiye

Publication: Damm et al. 2024 - https://doi.org/10.1021/acs.jproteome.4c00171

This DATASET collection includes the mass spectrometry files for proteomics venom investigation of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Türkiye.

Species list:

Vipera berus barani

Vipera darevskii

Montivipera bulgardaghica bulgardaghica 

Montivipera bulgardaghica albizona

Montivipera xanthina

Macrovipera lebetinus obtusa

Daboia palaestinae

Folders 01-07 - BOTTOM-UP PROTEOMICS: The venom pools were investigated by the bottom-up "snake venomics" (labled as SVX) approach and in short: separated by RP-HPLC, followed by SDS-PAGE separation and the single bands were in-gel processed by DTT, IAC and finally o/n tryptic digested. Samples submitted to HPLC-MS/MS. Early peptidic fractions of the first HPLC run were directly submitted to HPLC-MS/MS analytic w/o further gel procession. Folders 01 to 07 include the MS and MS/MS spectra of the snake species 1-7, respectively. Files are included as RAW and MZML format.

Used instrument: LTQ Orbitrap XL mass spectrometer (Thermo, Bremen, Germany) with an Agilent 1260 HPLC system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Grace Vydac 218MS C18 (2.1 × 150 mm; 5 μm particle size) column.

Modifications: UNIMOD:4 - \"Iodoacetamide derivative.\"

Used protein database: Uniprot_8570_serpentes_reviewed_canonical_2640_entries_cRAP_210408.fasta

Folders 10-11 - TOP-DOWN PROTEOMICS: The venom pools were investigated by the non-reduced and TCEP reduced top-down (labled as TD) approach and in short: untreated or TCEP reduced samples submitted to HPLC-MS/MS. Folders 10 and 11 include the MS and MS/MS spectra of the snake species 1-7 as labled. Files are included as RAW and MZML format.

Used instrument: Q Exactive HF mass spectrometer (Thermo, Bremen, Germany) with a Vanquish ultra-high performance liquid chromatography (UHPLC) system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Supelco Discovery BIO wide C18 (2.0 × 150 mm; 3 μm particle size; 300 Å pore size).

Modifications: none (either red. or non-red. disulfide bridges)

Used protein database for TopPIC analysis: Uniprot_8570_serpentes_reviewed_ISOandCAN_2749_entries_NOcRAP_231011.fasta