Each group of Myocardial Function in Isolated Ischemia/Reperfused hearts.

a. Baseline. b. Perfusion 30 min. c. Perfusion 120 min. Retrograde perfusion of the hearts was performed in a non-recirculating Langendorff apparatus with. A latex balloon was placed in the left ventricle via left atrium and connected to a computer coupled through a pressure transducer. The concentration of SAA (20 µM), PD (20 µM), SP (10 µM) used were chosen to performed the experiments. SAA+I/R group hearts were perfused with SAA for 30 min before I/R, for the PD+I/R group, hearts were pretreated with PD for 30 min following I/R, PD + SAA+IR group hearts were perfused with PD for 30 min, followed by the same procedure performed in the SAA+I/R group. *P<0.05. **P<0.01 versus CON group. $P<0.05. $$P<0.01 versus I/R #P<0.05 ##P<0.01 versus SAA+I/R &P<0.05 &&P<0.01 versus PD+SAA+I/R. All data were expressed as mean ±SEM, n = 6.