GNPS - In situ temporal changes in VOCS emission of Dendrobates auratus using PDMS films for in vivo sampling

Samples from extraction of volatile organic compounds from frogs using PDMS films and posterior thermal desorption using an incubation time of 20 min to 190C. Splitless injection using a syringe to 145C. Inhlet temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a Agilent 7200 GC-MS QTof (Agilent technologies, Palo Alto, CA, USA). Separation was performed HP-5MS capillary GC column (30 m 0.25 mm 0.25 um, Agilent technologies, Palo Alto, CA, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.