Meta-transcriptomic analysis reveals geographical expansion of known sugarbeet-infecting viruses and the occurrence of a novel virus in sugarbeet in the United States

Total RNA was extracted from the hairy-root tissue harvested from beet samples each representing a pool of hairy roots obtained from four to eight beets and from the symptomatic leaf samples each representing portions of leaves obtained from four to eight sugar beet plants from California, Colorado, Idaho, Minnesota, and North Dakota exhibiting symptoms resembling virus diseases and 18 libraries were prepared using Ribo minus rRNA depletion RNAseq.