Trancriptome analysis of tumor tissue to search for the associations between USP8 mutation status, molecular markers of pituitary adenomas, and clinical, and diagnostic parametres of Cushing disease

In this work we employed the comprehensive evaluation of clinical data of the cohort of CD patients along with the sequencing of 14 exon of USP8 to estimate the prevalence of USP8 mutations in our cohort and transcriptome analysis of tumor tissue to search for the associations between USP8 mutation status, molecular markers of pituitary adenomas, and clinical and diagnostic parameters of Cushing's syndrome including hormonal secretion, tumor size, aggressiveness, and SSRT2/SSRT5 expression. Overall design: The RNA were extracted from pituitary tumor brain tissue after transfenoidal surgery from alive patients. Under investigation were patients carrying the USP8 mutation and patients carrying the wild type of the gene.