Additional file 2: of Single-cell genomics of co-sorted Nanoarchaeota suggests novel putative host associations and diversification of proteins involved in symbiosis

Table S1. Read count, assembly statistics, completeness and contamination estimates from CheckM, and Nanoarchaeota-host pairing information for SAGs and selected Nanoarchaeota reference genomes. Table S2. Data identifiers for SAGs and reference genomes used in this study. Table S3. Assembly statistics, completeness and contamination estimates from CheckM, and additional information for reference genomes for comparison to putative host genome bins. Table S4. Assembly statistics, completeness and contamination estimates from CheckM, and additional information for Nanoarchaeota genome bins 95% ANI are highlighted. All cells with alignment lengths less than 20 kb have been set to 0% ANI. Table S7. Similarity of Nanoarchaeota 16S rRNA gene sequences extracted from genome bins and references. Sequences that are > 98% similar are highlighted in green, sequence lengths are shown in parentheses. Table S8. Average nucleotide identity (ANI) of putative host genome bins and host reference genomes. Comparisons > 95% ANI are highlighted. All cells with alignment lengths less than 20 kb have been set to 0% ANI. Table S9. Alignment results for proteins potentially horizontally transferred between hosts and Nanoarchaeota. Table S10. Within-SAG SNPs in single-sorted Nanoarchaeota SAGs and co-sorted Nanoarchaeota and putative host genome bins, at a MAF > 10%. Table S11. Simulated within-SAG SNPs for pooled datasets of 1 to 6 SAGs, at a MAF > 10%. Table S12. SNPs in all clade 1 Nanoarchaeota genome bins (> 100 kb bin size), mean values shown for each site type. Table S13. Pairwise comparisons of mean non-synonymous SNPs per kb between functional categories of genes in clade 1 Nanoarchaeota SAGs and SAG bins, from post-hoc Tukey HSD tests. Significant comparisons are highlighted in green. (XLSX 65 kb)