Microarray analysis of inflammatory gene expression during culture of CT26 tumor cells in macrophage conditioned buffer.

RAW 264.7 conditioned buffer was applied to CT26 cells for 24 hrs prior to mRNA isolation and analysis using the Codelink Mammalian Inflammation Bioarray. Significance of transcript upregulation or downregulation was determined using the VAMPIRE statistical algorithm. Data represents the fold change in transcript expression from 3 separate experiments. Of the 85 genes detected in CT26 cells (Supplemental Table S2), the most highly upregulated or downregulated genes associated with migration, proliferation and angiogenesis are presented.