DNA methylation data from renal cancer patient-derived xenograft tumor treated with temsirolimus or vehicle

We kept a primary xenograft models (KURC;Kyoto University Renal Cancer-3) derived from human renal cell carcinoma tissues, and 10 mg/kg of temsirolimus or vehicle was intraperitoneally administered.We performed DNA methylation analysis to compare the methylation profile of temsirolimus-treated primary xenograft tumors with that of vehicle-treated. We kept a patient-derived xenograft models (KURC3) derived from human renal cell carcinoma tissues. Ten mg/kg temsirolimus or vehicle only was intraperitoneally administered once a week.Once xenograft tumors had developed sufficiently, they were removed and transplanted into other SCID mice. Two weeks later, temsirolimus was re-administered to these mice, and this was repeated until tumors became completely resistant to temsirolimus KURC3 developed resistance to temsirolimus. DNA methylation profiling was performed for two KURC3 xenograft tumors using the Infinium HumanMethylation450 BeadChip array (Illumina Inc.).