p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation driven pancreatic cancer. p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation driven pancreatic cancer

To investigate the transcriptomic effects of iASPP deletion or p53 mutation in the KRASG12D background Pdx1-Cre was used to delete iASPP in oncogenic KRASG12D expressing (KC) background to generate KC;iASPPΔ8/Δ8 mice. KC and KC;iASPPΔ8/Δ8 mice were then crossed with mice containing a p53R172H allele (equivalent to human p53 hotspot mutation R175H) to generate KPC and KPC;iASPPΔ8/Δ8 cohorts. Primary cell cultures were then derived from pancreatic ductal adenocarcinoma tissue for RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for KC, KC;iASPPΔ8/Δ8,KC, and KC;iASPPΔ8/Δ8 mouse cell lines.