Single cell RNA-seq analysis of haemopoietic stem cells and CML stem cells isolated from human peripheral blood

To identify differences in the transcriptomes of normal haemopoietic stem cells (HSC) and leukaemic stem cells (LSC) found in patients with chronic myeloid leukaemia (chronic phase) we performed single cell RNA-seq. Overall design: Single cell RNA-seq was performed for HSC isolated from CD34+ cell samples obtained by mobilization with granulocyte-colony stimulating factor (G-CSF) from 5 unrelated individuals with no BM disease involvement. Single cell RNA-seq was performed on LSC isolated from CML CD34+ samples from 5 unrelated chronic phase patients that were randomly chosen without prior knowledge of age, sex, clinical outcome or response to tyrosine kinase inhibitors.