openDVP multiplex immunofluorescence demo

Processed: Illumination corrected with BASICStitching and Registration with ASHLARBackground subtraction with BackSubDownscaled to 8bit with DownscalerA small dataset of 5000 x 5000 pixels with 15 channels.Pixel size = 0.3467Microscope used: Axioscan7Tissue: Triple Negative Breast CancerObjective: 20X / NA0.5Binning: 2x2 Markers: cycle_number channel_number marker_name 1 1 750_bg 1 2 647_bg 1 3 555_bg 1 4 488_bg 1 5 DAPI_bg 2 6 Vimentin 2 7 CD3e 2 8 panCK 2 9 CD8 2 10 DAPI_1 3 11 COL1A1 3 12 CD20 3 13 CD68 3 14 Ki67 3 15 DAPI_2