Data from: IM-TORNADO: a tool for comparison of 16S reads from paired-end libraries

Motivation: 16S rDNA hypervariable tag sequencing has become the de facto
method for accessing microbial diversity. Illumina paired-end sequencing,
which produces two separate reads for each DNA fragment, has become the
platform of choice for this application. However, when the two reads do
not overlap, existing computational pipelines analyze data from read
separately and underutilize the information contained in the paired-end
reads. Results: We created a workflow known as Illinois Mayo Taxon
Organization from RNA Dataset Operations (IM-TORNADO) for processing
non-overlapping reads while retaining maximal information content. Using
synthetic mock datasets, we show that the use of both reads produced
answers with greater correlation to those from full length 16S rDNA when
looking at taxonomy, phylogeny, and beta-diversity. Availability and
Implementation: IM-TORNADO is freely available at
http://sourceforge.net/projects/imtornad​o and produces BIOM format output
for cross compatibility with other pipelines such as QIIME, mothur, and
phyloseq.