Dynamic transcriptome analysis of human erythroind progenetor cells infected by human Parvovirus B19

Human parvovirus B19 (B19V) infection can cause transient aplastic crisis, persistent viremia, and pure red-cell aplasia. In fetuses, B19V infection can result in non-immune hydrops fetalis and fetal death. To systematically investigate the interaction between B19V and erythoid progenetor cells (EPC), microarray was applied to systematically analyze the dynamic transcriptome of CD36+ EPCs during B19V infection. The mock- and B19V-infected CD36+ EPCs were collected at 6, 12, 24, and 48 hpi. RNA samples were extracted using the miRNeasy Mini Kit by following the manufacturer’s instruction. At each time points, 3 repeat samples were prepared. All RNA samples were analyzed on an Agilent 2100 Bioanalyzer with an Agilent RNA 6000 Nano Kit to ensure an RNA Intergrity Number (RIN) ≥ 8.0. Microarray hybridization was performed on the GeneChip Human Genome U133A 2.0 Array using the Affymetrix GeneChip system (Affymetrix, CA).