Transcriptional analysis of sodium valproate and lithium in a serotonergic cell line

Sodium valproate (VPA) and lithium are mood stabilizers, widely prescribed in the treatment of bipolar disorder, and yet the precise modes of therapeutic action for these drugs are not fully understood. After exposure of the rat serotonergic cell line RN46A to VPA, RNA-sequencing (RNA-Seq) analysis showed widespread changes in gene expression but no changes with lithium. Analysis by multiple pipelines revealed that as many as 230 genes were significantly upregulated and 72 genes were significantly downregulated in response to VPA exposure.丙戊酸钠（Sodium valproate，VPA）与锂盐均为心境稳定剂，被广泛用于双相情感障碍的临床治疗，然而这两类药物的确切治疗作用机制仍未完全阐明。将大鼠血清素能细胞系RN46A暴露于丙戊酸钠环境后，RNA测序（RNA-sequencing, RNA-Seq）分析显示其基因表达出现广泛改变；而经锂盐处理后则未观察到此类变化。通过多套分析管线验证发现，丙戊酸钠暴露组中共计有230个基因被显著上调，72个基因被显著下调。