Histone H3K4me3 signal breadth reveals constitutive and hypoxia stress responsive endometrial functions linked to endometriosis

We report sequening of chromatin immunoprecipitated DNA (ChIP-Seq) of histone H3 at lysine 4 (H3K4me3) normoxic and hypoxia treated endometrial stromal fibroblasts (ESF) and hypoxia treated differentiated decidual stromal cells (DSC), We conducted H3K4me3 ChIP-seq in hypoxia treated (16 h, 1% O2) undifferentiated endometrial stromal fibroblasts (ESF) and differentiated decidual stromal cells (DSC) and compared the H3K4me3 marks to those in normoxia. For DSC, before hypoxia treatment ESF were in vitro decidulized with cAMP and progesterone (MPA) for 2 days.