Sirtuin assay of purified Hos2 preparations using resveratrol: UPDATE 1

Fluorimetric Sirtuin1 like deacetylase assay with rHos2 protein using Fluor de Lys®−Sirt1 substrate in presence of Sirtuin1 activator Reserveratrol. This experiment is carried out in presence of HDAC inhibitor Trichostatin to inhibit any residual Histone deacetylase activity. Updated from: http://dx.doi.org/10.6084/m9.figshare.841658.

利用 rHos2 蛋白进行荧光量度 Sirtuin1 类似去乙酰化酶测定，采用 Fluor de Lys®−Sirt1 作为底物，并在 Sirtuin1 激活剂白藜芦醇的存在下进行。实验在 HDAC 抑制剂三尖杉酯碱的辅助下进行，以抑制残留的组蛋白去乙酰化酶活性。信息更新自：http://dx.doi.org/10.6084/m9.figshare.841658。