Mammary gland tumor immunoinfiltrate analysis

The project in which this experiment is included demostrate that the developtment and growth of breast tumors are under immunosurveillance. We perform as screaning of multiple nutritional interventions to observe the impact of each of them in the tumor incidence, tumor growth and overall survival of the animasls. We concluded that safe nutritional measures including nicotinamide (NAM) supplementation efficiently mediates immunotherapeutic effects against established transplantable BC. In particular, the aim of this experiment was to analyse the effect that NAM has in the immune cells that infiltrate the breast tumors, more specifically the TSA mammary gland tumors. Our findings identify NAM as a potential strategy for the prevention and treatment of HR+ BC. In this experiment we performed single cell RNAseq of the immunoinfiltrate of TSA mammary gland tumors that have been treated or not with NAM for 10 days.