Cetobacterium somerae strain:NK01 Genome sequencing and assembly. Cetobacterium somerae strain:NK01

The genus Cetobacterium is considered an important and dominant group of gut bacteria in many freshwater fish, and members of this genus contribute to anaerobic metabolism. We performed 16S rRNA sequencing of the intestinal mucosa of Nile tilapia (Oreochromis niloticus) and found that Cetobacterium existed widely in the foregut, midgut, and hindgut mucosa, and was successfully isolated from the gut of tilapia. We sequenced its whole genome and predicted it to be a novel candidate species of Cetobacterium. We found that the strain produced amino acids, including leucine, isoleucine, valine, glycine, alanine, phenylalanine, and proline. The strain can use starch, sucrose, maltose, glucose, and mannose and synthesize and utilize glycogen. This study contributes to the development of fish probiotics and to the aquaculture industry.